Sterol 27-hydroxylase in bile acid biosynthesis. Mechanism of oxidation of 5 beta-cholestane-3 alpha,7 alpha,12 alpha,27-tetrol into 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid
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چکیده
منابع مشابه
Biliary bile acids, bile alcohols, and sterols of Alligator mississippiensis.
Bile from Alligator mississippiensis was found to contain a mixture of more than twenty bile acids, bile alcohols, and neutral sterols. Bile acids and bile alcohols were purified by reversed-phase high performance liquid chromatography and thin-layer chromatography. Concentrations were measured by gas-liquid chromatography on 1% HiEFF-8BP and identifications were made by mass spectrometry. The ...
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Oxidation of side chain of 5 beta-cholestane-3 alpha,7 alpha,12 alpha-triol was studied in a patient with cerebrotendinous xanthomatosis (CTX) and in control subjects, using various subcellular fractions of liver homogenate and a method based on isotope dilution-mass spectrometry. In the control, 5 beta-cholestane-3 alpha,7 alpha,12 alpha-triol was converted into 5 beta-cholestane-3 alpha,7 alp...
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Bile salts of ten species of frogs of the family Ranidae were examined by means of thin-layer chromatography, gas-liquid chromatography, and gas-liquid chromatography-mass spectrometry. Their major bile alcohols were ranol, cyprinol, or bufol. Four species of frogs contained lesser amounts of higher bile acids, and in two species C24 bile acids were present. A higher bile acid that has not been...
متن کاملOccurrence of bile alcohols in the bile of a patient with cholestasis.
Considerable amounts (6.0 mumol/ml of bile) of bile alcohols were found in the bile of a patient with cholestasis due to gallstones in a common bile duct. Acid hydrolysis of the bile salts followed by chromatographic separation yielded three bile alcohols, which were identified as 26,27-dinor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol, 24-methyl-26,-27-dinor-5 beta-cholestane-3 alp...
متن کاملLiver mitochondrial P450 involved in cholesterol catabolism and vitamin D activation.
The isolation, purification, and cloning of the mitochondrial P450 enzyme catalyzing not only the 27-hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha-diol and cholestane-3 alpha, 7 alpha, 12 alpha-triol, but also the 25-hydroxylation of vitamin D3 are reviewed. The sterol hydroxylase was shown to be present on the mitochondrial inner membrane-matrix, to be inactivated by carbon monoxide, and...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)82094-4